Hydroxyproline Indices

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Contiguous hydroxyproline residues direct hydroxyproline arabinosylation in Nicotiana tabacum.

Hydroxyproline (Hyp) O-glycosylation characterizes the hydroxyproline-rich glycoprotein (HRGP) superfamily of the plant extracellular matrix. Hyp glycosylation occurs in two modes: Arabinosylation adds short oligoarabinosides (Hyp-arabinosides) while galactosylation leads to the addition of larger arabinogalactan polysaccharides (Hyp-polysaccharides). We hypothesize that sequence-dependent glyc...

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Stereoisomers of hydroxyproline.

Hydroxy-L-proline was first isolated by Fischer in 1902 from a hydrolysate of gelatin (l).l The synthesis and determination of the structure were reported by Leuchs and coworkers (5--g), and, in 1919, the separation of the two racemic stereoisomers and the resolution of each racemate through mixtures of quinine with the corresponding N-phenylisocyanate derivatives were accomplished (5). In the ...

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The determination of hydroxyproline.

There has been a great need for a satisfactory method for the determination of hydroxyproline to facilitate the study of the composition of proteins. Lang (1) and Waldschmidt-Leitz and Akabori (2) developed a calorimetric estimation involving oxidation to pyrrole with sodium hypochlorite and color formation with isatin or p-dimethylaminobenzaldehyde. However, oxidation was said to be incomplete...

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Determination of hydroxyproline in liver.

THE DETERMINATION of hydroxyproline in parenchymous tissues such as liver presents special problems not encountered in collagenons tissues. This is true because liver contains only small quantities of this imino acid (1), and furthermore, the existing quantitative tests are nonspecific. Thus, if one were to use the usually acceptable Neuman and Logan procedure (2), a separate determination for ...

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Hydroxyproline excretion in infantile gastroenteritis.

Total hydroxyproline/creatinine ratio (THP:Cr) was measured in random urine specimens from 18 infants with gastroenteritis. THP:Cr was sometimes increased during the acute phase of the illness and the reasons for this are discussed. Seven patients failed to thrive after the acute phase and THP:Cr was low in all of these. The test appears to be a sensitive indicator of the onset of failure to th...

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ژورنال

عنوان ژورنال: Nature

سال: 1967

ISSN: 0028-0836,1476-4687

DOI: 10.1038/215968a0